In situ multifunctional gel for hormone-immune therapy of ovarian cancer and prevention of postoperative adhesion

》》文章原文链接：In situ multifunctional gel for hormone-immune therapy of ovarian cancer and prevention of postoperative adhesion

》》Journal：Chemical Engineering Journal

》》相关产品： Tamoxifen (SJ-MX0142A)

》》产品引用描述：

》》Abstract：

Ovarian cancer is a significant risk to women’s health, whose cellular residues and infiltration are unavoidable in the postoperative period. In addition, postoperative adhesion (PA) provides a favorable environment for the growth and spread of residual cells, substantially increasing the risk of tumor recurrence. In this paper, an innovative treatment approach was developed to combine the prevention of postoperative adhesion and tumor recurrence. ID8 cell membrane was fused with tamoxifen-loaded liposome to create a biomimetic coating (MLips), which could circumvent chemotherapeutic toxicity and induce apoptosis in ovarian cancer cells. Hybrid MLips-coated siERK2-loaded protamine-hyaluronic acid nanoparticles (MLPHs) were developed to inhibit the aberrant activation of the MAPK pathway, prevent TAM resistance, and reverse immunosuppression. Mesenchymal stem cells (MSCs) were introduced to reduce the incidence severity of PA by utilizing their anti-inflammatory and mesothelial-to-mesenchymal transition (MMT). To maintain the MSCs activity and accurately deliver MLPH to the residual site, a postoperative gel in situ delivery strategy was adopted by building a delivery system co-loaded with MLPHs and MSCs (MLPHs/MSCs@Gel), which served as a physical barrier to effectively prevent adhesion formation, which also allowed for a controlled release of MLPHs. Thus, the multifunctional gel is a suitable delivery platform to improve tumor prognosis.

》》部分实验数据展示：

Fig. 4.  In vitro uptake, cytotoxicity, apoptosis assay, interference effects of siERK2 and anti-migration.

A) Cytotoxicity studies of TAM on ID8 and ID8-TR cells determined by CCK-8 assay for 24 h. (n = 5) B) The amounts of ERK2 mRNA on ID8 parent and resistant cells were measured without treatment by qRT-PCR method. (n = 3) ID8-TR viability following incubation with free TAM, siRNA, combo, NPs, Lips and MLPHs for C) 24 h and D) 48 h. (n = 5)