Kaempferol protects gut-vascular barrier from high glucose-induced disorder via NF-κB pathway

》》文章原文链接： Kaempferol protects gut-vascular barrier from high glucose-induced disorder via NF-κB pathway

》》Journal：J Nutr Biochem. 

》》相关产品：SN50 (SJ-MX2265) 

》》产品引用描述：

》》Abstract：

Kaempferol is a natural edible flavonoid reported to treat high-fat diet-induced intestinal inflammation; however, the underlying molecular mechanisms remain unclear. This research aims to investigate the protective effect of kaempferol on the gut-vascular barrier (GVB) induced by high glucose and elucidate the underlying mechanism. Evans blue albumin efflux assay was used to test endothelial cell permeability. The results showed that kaempferol (50 μM) significantly reversed the high glucose-induced monolayer barrier permeability of rat intestinal microvascular endothelial cells (RIMVECs), while kaempferol significantly alleviated the high glucose-induced rarefication of the tight junction protein Claudin-5. Moreover, kaempferol also reduced high glucose-induced angiogenesis and cell migration via inhibiting the VEGFR2/p38 pathway. Kaempferol also protected against high glucose-induced overproduction of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 by inhibiting NF-κB p65 nuclear translocation. In addition, kaempferol had similar effects to the NF-κB inhibitor SN50 in reducing high glucose-induced ICAM-1 expression and endothelial barrier permeabilization. Our findings in part reveal the pathological mechanism of hyperglycemia-related gastrointestinal diseases and underlie the molecular mechanism of kaempferol in inhibiting bowel inflammation from a novel perspective.

》》部分实验数据：

Fig. 1. The role of NF-κB in kaempferol prevention of endothelial barrier dysfunction induced by high glucose. (A) EB-albumin efflux in the RIMVECs monolayer barrier (n=4). (B) TER in the RIMVECs monolayer barrier (n=4). (C) The expression of soluble ICAM-1 in the cell culture supernatant was detected by ELISA (n=4). (D, E) The expression of ICAM-1 was detected by immunofluorescence (n=3). Scale bar = 50 μm. The results represent means ± SEM. ∗ P<.05; ∗∗ P<.01.